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. 2000 Jun 1;19(11):2475–2482. doi: 10.1093/emboj/19.11.2475

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graphic file with name cdd271f4.jpg — Fig. 4. Association of DPM1 and DPM2 with DPM3. (A and B) Human DPM3 was co-expressed in Lec15 cells with FLAG-tagged hDPM1 (lane 1), rDPM2 (lane 2), rDPM2FY/LS (lane 3) and hSL15 (lane 4), and their associations were analysed by immunoprecipitation. The lysates were immunoprecipitated with anti-FLAG (A) or anti-DPM3 (B). In (A), immunoprecipitates (middle and lower panels) and the supernatant (upper panel) were analysed by western blotting against anti-hDPM3 (upper and middle panels) and anti-FLAG (lower panel) antibodies. In (B), immunoprecipitates (middle and lower panels) and the supernatant (upper panel) were analysed by western blotting against anti-FLAG (upper and middle panels) and anti-hDPM3 (lower panel) antibodies. (C) GST-tagged human DPM2 was co-expressed in Lec15 cells with FLAG-tagged hSL15 (lane 1), hDPM3 (lane 2) and hDPM3dC (lane 3). Their lysates were immunoprecipitated with anti-FLAG beads. Precipitates (middle and lower panels) and the supernatant (upper panel) were analysed by western blotting against anti-GST (upper and middle panels) and anti-FLAG (lower panel) antibodies.