Figure 2.

Inverse correlation between GS induction and LDH release. (A) Cortisol (1 mg per egg) was injected into E15, E16, or E17 eggs, which were then incubated for an additional 48, 24, or 4 h, respectively. Retinal tissue was excised, cut into pieces, and organ-cultured for 4 h. The levels of GS in the tissue and of LDH in the culture medium were measured. (B) E15 eggs were injected with cortisol in the indicated amounts and incubated for an additional 48 h. Retinal tissues were excised, cut into pieces, and organ-cultured for 4 h. The level of GS in the tissue and of LDH in the culture medium was measured. (C) E15 eggs were coinjected with cortisol (1 mg per egg) and MSO (1 mg per egg), buthionine sulfoximine (BSO; 1 mg per egg), or carrier. The eggs were incubated for an additional 48 h before the retina was excised. Retinal explants were cut into pieces and organ-cultured for 4 h. The levels of GS in the tissue and of LDH in the culture medium were measured. The levels of GS and LDH obtain after coinjection of cortisol and carrier were given the arbitrary value of 100. Results are means ± SD of three independent experiments, each performed in duplicate (n = 6).