Figure 6.

Morphological evidence for protective effects of the purified GS enzyme. E17 eggs were injected with purified GS enzyme (2 units per egg; A and D) or with carrier (B and E) and incubated for 2 h before the retinas were excised. Retinal explants were embedded immediately after excision (A and B) or were cut into pieces, organ-cultured for 4 h, and then embedded in paraffin (D and E). Paraffin sections were stained with rabbit anti-GS-specific antiserum and with goat anti-rabbit IgG (A and B) or with hematoxylin/eosin (D and E). E15 eggs were injected with cortisol (1 mg per egg) and incubated for an additional 48 h before the retina was excised. The excised tissue was embedded in paraffin, and histological sections were stained with rabbit anti-GS-specific antiserum and with goat anti-rabbit IgG (C). Untreated E17 retina was embedded immediately after excision and stained with hematoxylin/eosin (F). (Bar = 25 μm.)