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Large Shear of the G–U wobble base pair influences the calculated but not the ‘observed’ Twist. The 3DNA numerical values of Twist, 20° (top) and 43° (bottom), differ from the visualization of nearly equivalent Twist suggested by the angle between successive C1′···C1′ vectors (finely dotted lines). Illustrated dimer steps flank the G(8)–U(12) base pair in the crystal structure of the acceptor stem of E.coli tRNAAsp (NDB_ID: ar0019) (60).
