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. 2003 Sep 1;31(17):5175–5185. doi: 10.1093/nar/gkg693

Figure 3.

Figure 3

Topology of the MFP1–thylakoid association. The thylakoid fraction was incubated with thermolysin in a time course as indicated. Proteins were resolved by 10% SDS–PAGE and probed by immunoblotting with the anti-LeMFP1 antibody 288 (A) or anti-OEC33 (B). C, total chloroplast protein; Th, thylakoid protein fraction; S, stroma and envelope protein fraction; Therm., thermolysin; 0, 2, 10, 30, incubation times in minutes. Molecular masses in kilodaltons (kDa) are indicated on the right in (A).