Figure 1.

Molecular characterization of the Hand¹⁷³ deletion mutation. A) Genomic map of the Hand gene region. Genes are shown in blue and the exons of the Hand gene are shown in pink underneath. The location of the insertion of the GE10558 EP element is indicated at the top, and the endpoints of the imprecise excision deletion of Hand¹⁷³ are denoted by the brackets at the bottom. B) Nucleotide sequence of the genomic DNA bordering the deletion of Hand¹⁷³. The 23 bp sequence which replaces 1.2 kb of Hand sequences is highlighted by the red box. Sequence numbering is relative to the adenosine of the ATG start codon of the Hand open reading frame. C) Dorsal views of a control (left) and Hand¹⁷³ mutant (right) embryo stained for Hand mRNA in situ (green) and anti-MEF2 and anti-β-gal (red). The Hand¹⁷³/CyO control embryo expresses striped wg-lacZ from the balancer chromosome.