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Reduction of HIV-1 p24 antigen in cell-free supernatant and cell lysate determined by western blot analysis. 293T cells were co-transfected with HIV-1NL4.3 and the different shRNA expression vectors. Non- transfected 293T cells served as a negative control. The control plasmid MTD-luc expresses a luciferase shRNA. Tubulin served as a loading control.
