Table 1.
UV-induced mutations in wild-type, rad30 and rev3 yeast strains.
| rad30Δ | ||||||||
|---|---|---|---|---|---|---|---|---|
| wild-type |
rev3-L979F |
rad30Δ |
rev3-L979F |
|||||
| Freq. (×10−6) |
88 [59 - 120] |
200 [50 - 350] |
200 [170 - 230] |
540 [470 - 590] |
||||
| Events | Freq. | Events | Freq. | Events | Freq. | Events | Freq. | |
| A-T to G-C | 4 | 14 | 0 | 0 | 2 | 16 | 0 | 0 |
| G-C to A-T | 8 | 28 | 6 | 48 | 12 | 92c | 10 | 220c |
| A-T to T-A | 6 | 21 | 2 | 16 | 4 | 32 | 2 | 43 |
| A-T to C-G | 0 | 0 | 0 | 0 | 1 | 8 | 0 | 0 |
| G-C to T-A | 1 | 4 | 2 | 16 | 2 | 16 | 4 | 86c |
| Tandem bps | 1 | 4 | 3 | 24 | 0 | 0 | 4 | 86c |
| Indelsa | 3 | 11 | 4 | 32 | 0 | 0 | 2 | 43 |
| Complexb | 2 | 7 | 8 | 64c | 5 | 48 | 3 | 65 |
Cells were exposed to 20 J/m2 of UVC.
Freq. represents mutation frequency. All mutation frequencies are (×10−6). Numbers in brackets represent one standard deviation.
a
One and two-base insertions and deletions were categorized as indels.
b
Multiple, closely spaced base substitutions and/or indels were categorized as complex mutations. One of the can1 isolates from the rad30Δ strain contained three single base changes within 30 base-pairs.
c
Significant difference (p < 0.05) from the REV3 strain. P-values were determined as described in the materials and methods.