FIG. 3.
Glucose transport and glycolysis are required for glucose-induced activation of KATP channels. Membrane currents in the absence and presence of glucose (50 mmol/l; 4 min of exposure) were recorded using perforated patch-clamp electrophysiology in cardiomyocytes pretreated with 8-p-CPT-cGMP (200 μmol/l) or 2-deoxyglucose (50 mmol/l). The bar graph shows current density at +80 mV in cells under depicted conditions and time points. Time points refer to the time from the moment of establishing the whole-cell configuration. Each bar represents the means ± SE (n = 4-6). *P < 0.01 when compared with the control.