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. Author manuscript; available in PMC: 2007 Dec 10.

Published in final edited form as: Diabetes. 2005 Feb;54(2):383–393. doi: 10.2337/diabetes.54.2.383

FIG. 4 — Intracellular glucose has no effect on whole-cell K⁺ current measured by conventional patch-clamp electrophysiology. A and B: Membrane currents recorded in response to 400 ms-long pulses (from -100 to +80 mV) using conventional whole-cell patch-clamp electrophysiology (this technique is schematically presented on the figure as a rectangular shape (depicting a cardiomyocyte) in contact with a tip of the patch pipette while a part of the sarcolemma is disrupted, allowing pipette solution to replace cytosol) and corresponding I-V relationships (each point is the means ± SE, n = 6-11) at depicted time points. Cardiomyocytes were filled up with pipette solution without (A) or with (B) glucose (20 mmol/l). Time point 0 min refers to the recording made immediately after the whole-cell configuration was established. C: Current density at +80 mV under conditions in A and B. Each bar represents the means ± SE (n = 6-11).

FIG. 4 — Intracellular glucose has no effect on whole-cell K⁺ current measured by conventional patch-clamp electrophysiology. A and B: Membrane currents recorded in response to 400 ms-long pulses (from -100 to +80 mV) using conventional whole-cell patch-clamp electrophysiology (this technique is schematically presented on the figure as a rectangular shape (depicting a cardiomyocyte) in contact with a tip of the patch pipette while a part of the sarcolemma is disrupted, allowing pipette solution to replace cytosol) and corresponding I-V relationships (each point is the means ± SE, n = 6-11) at depicted time points. Cardiomyocytes were filled up with pipette solution without (A) or with (B) glucose (20 mmol/l). Time point 0 min refers to the recording made immediately after the whole-cell configuration was established. C: Current density at +80 mV under conditions in A and B. Each bar represents the means ± SE (n = 6-11).