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. 2003 Sep 1;31(17):4973–4980. doi: 10.1093/nar/gkg707

Figure 3.

Figure 3

Altering the miR-13 target site in the 3′ UTR of gene CG10222 and its influence on gene expression. (Left) The 3′ UTR of gene CG10222 and three mutant constructs with its predicted base pairing with miR-13b. The 3′ UTRs were fused to a pact-GL3 reporter construct and transfected into S2 cells. Transfection efficiency was first normalised to a reporter plasmid expressing LacZ. (Right) Relative expression level in percent with respect to the wild-type 3′ UTR of gene CG1022, which was set to 100%. Average expression levels and standard deviations obtained from six independent experiments with 1800 (left bars) or 450 ng (right bars) are given. The relative effect of the mutations is stronger with the lower amount of transfected plasmid.