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. 2003 Sep 1;31(17):5048–5053. doi: 10.1093/nar/gkg709

Figure 2.

Figure 2

Polymerase extension through consecutive non-standard bases. A cyanine 5 (CY5) 5′-labeled DNA primer hybridized to a complementary DNA template was extended by Taq DNA polymerase in the presence of various non-standard and standard deoxynucleoside triphosphates. Extension products were resolved by denaturing polyacrylamide gel electrophoresis and detected by fluorescence imaging. Deoxynucleoside triphosphate combinations are indicated on top of each lane. Lane 1, no triphosphate (–); lane 2, d-isoCTP (iC); lane 3, d-isoCTP + d-isoGTP (iC,iG); lane 4, d-isoCTP + d-isoGTP + dGTP (iC,iG,G); lane 5, d-isoCTP + d-isoGTP + dNTP, where N = A, C, G, T (All); lane 6, d-isoCTP + dNTP (-iG). Reaction products are diagrammed on the left and are lined up with their respective band size.