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. 2003 Sep 1;31(17):5186–5194. doi: 10.1093/nar/gkg714

Figure 3.

Figure 3

DNA and tRNAlys3 primer binding to PBS-mutated HIV-1 transcripts. (A) Electrophoretic analysis of vRNA–primer complexes formed in the presence of a DNA primer (lanes 1) and tRNAlys3 (lanes 2). Control samples include HIV-1 RNA without primer (lanes 3) and formamide-denatured samples (lanes 4). The number of PBS nucleotides is indicated at the top of the gel for each transcript. (B) Quantified data of the gel shown in (A), showing the yield of vRNA–primer complexes with the DNA primer or tRNAlys3.