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. 2003 Sep 1;31(17):5054–5063. doi: 10.1093/nar/gkg718

Figure 2.

Figure 2

Rearrangement of TAS1 in pku80-d cells is Rhp51 dependent but not Rad50 dependent. Freshly prepared pku80-d cells, which were constructed by one-step gene replacement (corresponding to ∼42 generations), were crossed with wild-type haploid cells (JY741) (lane 2, TK005; lane 3, TK006; lane 4, TK007), crossed with rhp51-d cells (KT10c) (lane 5, TK012; lane 6, TK013; lane 7, TK014), crossed with rad22-d cells (KT10b) (lane 8, TK015; lane 9, TK016) or crossed with rad50-d cells (KT102) (lane 10, TK009; lane 11, TK010; lane 12, TK011) and grown for ∼168 generations on YPAD plates. Genomic DNA was analyzed as described in Figure 1B. Lane 1, wild-type h– strain (JY741); lane 13, wild-type h+ strain (JY746). The position of the aberrant band ladder is shown by the bracket. The ∼6 kb band in lane 5 shown by an asterisk came from h+ rhp51-d cells.