Figure 2.

Effects of peptides on thymidine incorporation by embryonic (A and B) or adult (C) hippocampal NPCs. (A) Although peptide P2 had no effects by itself, it inhibited thymidine uptake by embryonic NPCs in a dose-dependent manner. (B) Both peptides P2 and P4 inhibited the mitogenic effects of FGF-2 and FGF-4, but peptides P1, P2–2 or P5 had no effect (P + FGF-2 or P + FGF-4). Addition of FGF-2 before the addition of peptides P2 and P4 (FGF-2 + P2 or P4) abolished their inhibitory effects, indicating that the peptides are not toxic. (C) Peptides showed similar effects on cultured adult hippocampal NPCs. Addition of FGF-2 before peptides P2 or P4 (FGF-2 + P2 or P4) abolished their inhibitory effects.