Figure 4.

Inhibition of ¹²⁵I-FGF-2 affinity labeling by peptides. (Upper) Adult hippocampal NPCs were treated with peptides P2, P4, or P5 before the addition of radiolabeled FGF-2. The molecular mass markers are indicated on the left. Arrowheads on the right indicate the two receptor polypeptides present in NPCs. The bands appear to be 150 and 100 kDa. Allowing for the molecular mass of FGF-2, the molecular masses of these proteins appear to be about 135 and 85 kDa, respectively. (Lower) The panel of peptides was examined for their ability to inhibit receptor affinity labeling on BHK cells. The bands appear to be 150- and 130-kDa doublet (arrowheads). Peptides P2 and P4 were more effective in blocking of the binding of FGF-2 on both of the receptors, whereas peptide P5 showed lower inhibitory effects.