Figure 3.
Cellular responses to GIRK activation at physiological [K+]o. (Left) Currents activated by 30 μM 5-HT and 50 μM baclofen in AdGIRK1+2-coinfected (4 d postinfection, thick lines) and uninfected (fine line, 5-HT only) hippocampal neurons 11 d in culture (Upper), and by 5 μM ACh in an atrial cell (Lower). (Right) EM changes caused by application of 50 μM baclofen and 5 μM ACh (bars); resting EM were −68 (neuron) and −24 mV (myocyte). Similar responses were observed to 8-OH-DPAT in neurons. The mean resting EM of 15 atrial cells was −35.8 ± 3.8 mV.