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In ocular sebaceous carcinoma, when fresh tissue is available, oil red-O stain can vividly reveal intracytoplasmic lipid when findings are equivocal, particularly on frozen section (A, B). More recently, adipophilin has been demonstrated to detect sebaceous differentiation in permanent sections where lipid has been extracted during tissue processing (C, D) (courtesy of Dr. Fiona Roberts, Glasgow, UK).
