Skip to main content
. Author manuscript; available in PMC: 2007 Dec 10.
Published in final edited form as: FASEB J. 2006 Oct 31;20(14):2556–2558. doi: 10.1096/fj.06-6252fje

Figure 6.

Figure 6

Preconditioning phosphorylates PKB in PDK1fl/+ hearts and inhibits dephosphorylation of GSK-3β. The effect of ischemia on phosphorylation of PKB and GSK-3β. Original Western blots with total PKB (A), phospho-PKB (B), total GSK-3β (C), and phospho-GSK-3β (D) antibodies applied on extracts from hearts under control conditions (control), and those exposed to 20-min-long ischemia without preconditioning (ischemia), to four cycles of ischemia/reperfusion (time 0 in preconditioning), 20-min-long ischemia after the preconditioning (ischemia after preconditioning) and treated with insulin (insulin), and corresponding graphs. Each bar represents mean ± sem. *P < 0.05 (as determined by ANOVA followed by t test).