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. Author manuscript; available in PMC: 2008 Nov 7.
Published in final edited form as: Vaccine. 2007 Sep 4;25(45):7824–7831. doi: 10.1016/j.vaccine.2007.08.036

Figure 2. Flow cytometry analysis of the E6- or OVA-specific CD8+ T cell responses in mice vaccinated with combinations of SCT DNA and Bcl-xL or mtBcl-xL DNA vaccines.

Figure 2

C57BL/6 mice were vaccinated intradermally via gene gun with pIRES-E6-β2m-Kb or pIRES-OVA-β2m-Kb combined with pSG5-Bcl-xL or pSG5-mtBcl-xL. Splenocytes obtained from vaccinated mice were cultured with E6 peptide (aa49-57) or OVA peptide (aa257-264) overnight, followed by analysis for CD8 and intracellular IFN-γ staining by flow cytometry. A) Representative flow cytometry data showing the number of IFN-γ-producing E6- or OVA-specific CD8+ T cells in vaccinated mice. Data shown are representative of two separate analyses. B) Bar graph depicting the average number of IFN-γ+ CD8+ T cells from each group of vaccinated mice (*p<0.05).