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. Author manuscript; available in PMC: 2008 Sep 10.
Published in final edited form as: Exp Cell Res. 2007 Jun 27;313(15):3319–3325. doi: 10.1016/j.yexcr.2007.06.006

Fig 5.

Fig 5

Transactivation of the EGFR is required for induction of a fully motile phenotype in MDCK cells. (A) Activation of the EGFR in MDCK cells under conditions similar to those shown in Fig. 1B except that 20 ng/ml HGF was used. The signals were significantly reduced in the presence of tyrphostin AG1478 and GM6001 (*, P< 0.0005; **, P< 0.001) (B) Phase contrast photo-micrographs of control MDCK colonies, colonies stimulated with 20 ng/ml HGF for 7 hours, and colonies stimulated with HGF in the presence of 10 μM tyrphostin AG 1478 (tyr). (C) Cells were stained with Alexa Fluor® 546-labeled phalloidin. (D) Wounding assay was performed as in Fig. 3B. The means of quadruplicate measurements are shown and the error bars are standard deviations.