Table I.
Amniotic fluid cytokine and prostaglandin concentrations before and after experimental intra-amniotic GBS infection without treatment, treatment with ampicillin (AMP) alone, or treatment with ampicillin+dexamethasone+indomethacin (AMP/DEX/INDO)
| All (n=15) | No Treatment (n=6) | AMP Alone (n=4) | AMP/DEX/INDO (n=5) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Pre-Infection Basal |
Peak Concentration† |
Delivery |
p value* |
Pre-treatment Concentration† |
Post-treatmen t Concentration |
p value* |
Pre-treatment Concentration† |
Post-treatment Concentration |
p value* |
|
| IL-1β | 0.14 ±0.07 | 2.48 ± 0.49 | 2.53 ±0.76 | 0.72 | 2.09 ± 0.65 | 1.42 ± 0.56 | 0.18 | 5.01 ± 1.42 | 0.59 ± 0.23 | 0.06 |
| TNF-α | 0.02 ±0.01 | 3.47 ± 0.17 | 5.2 ±0.23 | 0.11 | 2.64 ± 1.08 | 1.22 ± 0.61 | 0.29 | 0.94 ± 0.51 | 0.08 ± 0.01 | 0.04 |
| IL-6 | 16.3 ±5.0 | 196.6 ± 99.6 | 116.6 ±47.6 | 0.11 | 259.6 ± 76.7 | 249.0 ± 121.1 | 0.59 | 479.0 ± 93.2 | 144.6 ± 38.8 | 0.04 |
| IL-8 | 2.8 ± 1.7 | 177.9 ± 62.1 | 166.7 ±73.3 | 0.32 | 191.0 ± 16.3 | 177.7 ± 19.4 | 0.11 | 130.5 ± 30.4 | 119.9 ± 28.5 | 0.68 |
| PGE2 | 0.54 ±0.20 | 16.6 ± 3.53 | 8.84 ±2.89 | 0.11 | 22.63 ± 12.01 | 11.32 ± 4.26 | 0.11 | 9.17 ± 3.18 | 0.18 ± 0.04 | 0.04 |
| PGF2α | 0.22 ±0.06 | 5.14 ± 1.86 | 3.73 ±1.63 | 0.47 | 2.63 ± 0.22 | 1.66 ± 0.09 | 0.11 | 3.31 ±1.20 | 0.14 ± 0.04* | 0.04 |
Concentrations are in nanogram/milliliter and presented as mean ± standard error of the mean (ng/ml).
†
All pre-treatment peak cytokine and prostaglandin concentrations were statistically significantly higher than basal levels (p<0.05) by Wilcoxin signed ranks test.
*
Wilcoxon signed ranks test, post-treatment versus pre-treatment within each treatment group.