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Relative abundance of mRNAs synthesized from genes PIR1, PIR2, and PIR3. Five micrograms of total RNA purified from BWG7a (lanes 1 and 2) and GRF167 (lanes 3 and 4) cells growing at 30°C (lanes 1 and 3) or transferred to 37°C for 60 min (lanes 2 and 4) were hybridized to DNA probes corresponding to the open reading frames of the PIR1, PIR2, and PIR3 genes. Lane 5 shows RNA extracted from YAT1588 cells transformed with plasmid p414GPD carrying the indicated PIR gene.
