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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Vascul Pharmacol. 2007 Aug 9;47(5-6):257–264. doi: 10.1016/j.vph.2007.07.001

Fig. 4. Effects of Asp mutation of Ser-116 of eNOS on ACh-induced vasorelaxation in aortic rings from eNOS knockout mice.

Fig. 4

eNOS knockout mice were anesthetized with ketamine/xylazine and thoracic aortae were then injected with WT or 116D adenoviruses and incubated in DMEM serum-free media at 37°C overnight. Vessel segments were contracted with an EC50 concentration of phenylephrine. When the contraction reached a plateau, an accumulated dose response to ACh was conducted. The results shown are representative of three separate experiments (means ± S.E., n=3, * P < 0.05 vs. WT).