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Detection of DNA-2 replicative forms in TLCV-infected tomato. Identification of open circular dsDNA (oc), linear dsDNA (lin), supercoiled dsDNA (sc) and ssDNA (ss) forms was confirmed by digestion of total nucleic acid extracts with NcoI that linearized the oc and sc forms but had no effect on the ssDNA (compare lanes 2 and 3), and blotting of samples under nondenaturing conditions with 10 × SSC resulting in specific detection of ssDNA but not dsDNA (compare lanes 2 and 4).
