Figure 5.

Role of TLCV gene products in replication and encapsidation of TLCV sat-DNA. A, Genome organization of TLCV. Functional ORFs are displayed as thick arrows. The position of the conserved stem–loop structure (|○), intergenic region (IR), and the putative ori (↔) are marked. B, Southern blot of total nucleic acid extracts of tobacco leaf strips coagroinoculated with a dimeric TLCV sat-DNA construct and various TLCV ORF mutant constructs. Replicative DNA forms are labeled as in Fig. 2. High M_r DNA detected at the top of each blot represents inoculum (inoc) DNA isolated from residual A. tumefaciens on the leaf strips at the time of extraction. Note the TLCV V1/V2 mutant involves a 510-nt deletion resulting in the smaller sc and ssDNA forms observed in lane 7.