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. 1997 Jun 24;94(13):7088–7093. doi: 10.1073/pnas.94.13.7088

Figure 7.

Figure 7

Identification of putative sequences involved in TLCV-supported satellite replication. Putative stem–loop structures (|○) are shown in expanded form to show the conserved geminiviral sequence motif in stem–loop I (nt 1–9, boldface) and the putative TLCV Rep-binding motif in stem–loop II (nt 391–400, boldface). Introduced restriction sites generated by site-directed mutagensis are boxed. Letters A–E refer to regions deleted or inverted in mutant satellite constructs as described in the text. Inset (Top) shows a region of sequence conservation between TLCV sat-DNA and the TLCV as detected by DOTPLOT analysis. Inverted repeats forming a putative stem–loop structure are shown with arrows. The putative Rep binding motif (25) within the TLCV intergenic region is boxed.