Figure 2.

Serotonin immunoreactivity and the effect of serotonin depletion on the CSDn. (a-d) Staining using anti-serotonin antibodies (red) in different regions of the CSDn (green); (a) cell body, (b) contralateral terminals, (c) branches in the ipsilateral and (d) contralateral higher centers (arrows highlight puncta of serotonin immunoreactivity). (e-h) ddc^ts2/ddc^ts2; RN2-Flp, Tub-FRT-CD2-FRTGal4, UAS-mCD8GFP/+ animals grown at restrictive temperature show a strong reduction in serotonin immunoreactivity within the antennal lobe (e). The presynaptic terminals from a single CSDn (f), as well as the ipsilateral (g) and contralateral (h) arbors over the higher centers (arrowheads) do not show any appreciable change in branching pattern. Scale bar in all figures = 10 μm