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pH-dependence of phosphate uptake of transgenic tobacco cells. Cultured cells carrying either p35SPHT1 (•) or the vector pMi12 alone (○) were washed four times with phosphate-free medium (see Materials and Methods) of different pH (adjusted by Tris). Just before the phosphate uptake experiments, pH in each medium was measured. Data are the mean ± SD from three experiments.
