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. 2007 Dec 19;2(12):e1338. doi: 10.1371/journal.pone.0001338

Figure 5. Loss of Id1 does not affect function of pro-angiogenic myeloid cells.

Figure 5

Flow cytometry analysis of the TEMs in BM (A) peripheral blood (B) and LLC tumor (C) of wild type (wt) or Id1-/- mice. The plots represent the percentage of Tie2+ CD45+ Cd11b+ of nucleated cells in wild type and Id1-/- mice. Average±SEM: BM WT = 2.64±0.27 n = 9; Id1-/- = 2.5±0.17 n = 12. Peripheral blood WT = 2.1±0.21 n = 14; Id1-/- = 3.14±0.28 n = 14. Tumor WT = 2±0.27; Id1-/- = 1.44±0.16. D) Anti-CD68 immunofluorescence on LLC tumor sections showing infiltration of TAMs in WT and Id1-/- mice. Scale bar 50 µm. E) Anti-neutrophils antibody clone 7/4 immunofluorescence on LLC tumor sections showing infiltration of neutrophils in WT and Id1-/- mice. Scale bar 100 µm. F–G) Quantification of immunofluorescences shown in D and E. The histograms represent the number of positive cells counted per field (magnification 630X) in LLC tumors collected from WT and Id1-/- mice. A minimum of 6 non sequential sections were counted. TAM average±SEM (20.86±3.6 WT 23.67±4.5 Id1-/-) Neutrophils average±SEM (9.2±1.1 WT 10.7±1.2 Id1-/-).