Table 4.
The Spectra of Erythromycin Resistant Mutations
| % with nucleotide substitutions (no.)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1950
|
1951
|
1952
|
1949–1950
|
3993
|
|||||
| Strains | G→T | G→A | A→T | A→G | A→C | A→T | A→G | G ins | C→G |
| Spontaneous | |||||||||
| Wild-type | 5.6 (2) | 11.1 (4) | 33.3 (12) | 27.8 (10) | 2.7 (1) | 5.6 (2) | 5.6 (2) | 8.3 (3) | 0 |
| rev1-Δ | 0 | 5.0 (2) | 35.0 (14) | 32.5 (13) | 10.0 (4) | 0 | 10.0 (4) | 2.5 (1) | 5.0 (2) |
| rev3-Δ | 0 | 20.8 (5) | 37.5 (9) | 33.3 (8) | 0 | 4.2 (1) | 0 | 4.2 (1) | 0 |
| rev7-Δ | 0 | 0 | 28.6 (4) | 50.0 (7) | 7.1 (1) | 7.1 (1) | 7.1 (1) | 0 | 0 |
| UV-Induced | |||||||||
| Wild-type | 0 | 0 | 35.0 (7) | 55.0 (11) | 5.0 (1) | 0 | 5.0 (1) | 0 | 0 |
| rev1-Δ | 0 | 0 | 75.9 (22) | 13.8 (4) | 6.9 (2) | 3.4 (1) | 0 | 0 | 0 |
| rev3-Δ | 0 | 0 | 53.8 (14) | 26.9 (7) | 7.7 (2) | 0 | 7.7 (2) | 3.8 (1) | 0 |
| rev7-Δ | 0 | 0 | 20.0 (1) | 60.0 (3) | 0 | 0 | 0 | 20.0 (1) | 0 |
*
A total of 194 independent erythromycin resistant colonies were sequenced. Only two mutations were found at position 3993. These were: rev1-Δ C → G (2).