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. 1984 Aug;93(1):1–8. doi: 10.1017/s0022172400060861

Occurrence of a nonplasmid-located determinant for gentamicin resistance in strains of Staphylococcus aureus.

W Witte, K Dünnhaupt
PMCID: PMC2129281  PMID: 6235270

Abstract

The occurrence of resistance to gentamicin in strains of Staphylococcus aureus with different phage patterns in two hospitals is described. The data suggest transfer of gentamicin resistance between different strains. In the strains investigated the determinant for resistance to gentamicin (parallel resistance to kanamycin and tobramycin) is located on the chromosome. The transfer of this resistance determinant in a mixed culture of donor and recipient cells in vitro is mediated by transduction because it is dependent upon lysogenicity of the donor for a serogroup-B phage and because it can be inhibited by gamma-globulin. The transfer is always associated with lysogenization of the recipient cells by this phage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asheshov E. H. The genetics of tetracycline resistance in Staphylococcus aureus. J Gen Microbiol. 1975 May;88(1):132–140. doi: 10.1099/00221287-88-1-132. [DOI] [PubMed] [Google Scholar]
  2. Bint A. J., George R. H., Healing D. E., Wise R., Davies M. An outbreak of infection caused by a gentamicin-resistant Staphylococcus aureus. J Clin Pathol. 1977 Feb;30(2):165–167. doi: 10.1136/jcp.30.2.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buckwold F. J., Albritton W. L., Ronald A. R., Lertzman J., Henriksen R. Investigations of the occurrence of gentamicin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1979 Feb;15(2):152–156. doi: 10.1128/aac.15.2.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Greenhood G. P., Hill D. L., Dixon R. E., Carter M. J., Kanto W. P. Changing phage typing patterns of epidemic gentamicin-resistant Staphylococcus aureus. Evidence for transmission of gentamicin resistance. Lancet. 1979 Feb 10;1(8111):289–291. doi: 10.1016/s0140-6736(79)90703-7. [DOI] [PubMed] [Google Scholar]
  6. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kayser F. H., Homberger F., Devaud M. Aminocyclitol-modifying enzymes specified by chromosomal genes in Staphylococcus aureus. Antimicrob Agents Chemother. 1981 May;19(5):766–772. doi: 10.1128/aac.19.5.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lacey R. W. Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. Bacteriol Rev. 1975 Mar;39(1):1–32. doi: 10.1128/br.39.1.1-32.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lacey R. W., Lord V. L. Transfer of gentamicin resistance between cultures of Staphylococcus aureus in nutrient broth, serum and urine. J Med Microbiol. 1980 Aug;13(3):411–421. doi: 10.1099/00222615-13-3-411. [DOI] [PubMed] [Google Scholar]
  10. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Naidoo J., Noble W. C. Transfer of gentamicin resistance between strains of Staphylococcus aureus on skin. J Gen Microbiol. 1978 Aug;107(2):391–393. doi: 10.1099/00221287-107-2-391. [DOI] [PubMed] [Google Scholar]
  12. Nordström K., Forsgren A., Cox P. Prevention of bacteriophage adsorption to Staphylococcus aureus by immunoglobulin G. J Virol. 1974 Aug;14(2):203–206. doi: 10.1128/jvi.14.2.203-206.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. doi: 10.1016/0042-6822(67)90105-5. [DOI] [PubMed] [Google Scholar]
  14. Rosendal K., Bang J., Rosdahl V. T. Gentamicin-resistant Staphylococcus aureus strains isolated in Denmark in 1979. Acta Pathol Microbiol Scand B. 1981 Jun;89(3):185–191. doi: 10.1111/j.1699-0463.1981.tb00174_89b.x. [DOI] [PubMed] [Google Scholar]
  15. Warren R. E., Roberts S. O. Letter: Gentamicin-resistant Staphylococci. Lancet. 1976 Mar 6;1(7958):543–544. doi: 10.1016/s0140-6736(76)90336-6. [DOI] [PubMed] [Google Scholar]
  16. Witte W., Dünnhaupt K. Multiple drug-resistant Staphylococcus aureus with resistance to oxytetracycline and minocycline, an example for the distribution of a multiresistant strain-clone. J Hyg Epidemiol Microbiol Immunol. 1982;26(1):49–56. [PubMed] [Google Scholar]
  17. Witte W. Restriktion und Modifikation bei Staphylococcus aureus: Eigenschaften von Resistenzplasmiden und Prophagen. Z Allg Mikrobiol. 1976;16(6):405–474. [PubMed] [Google Scholar]
  18. Witte W. Transfer of drug-resistance-plasmids in mixed cultures of Staphylococci. Zentralbl Bakteriol Orig A. 1977;237(2-3):147–159. [PubMed] [Google Scholar]
  19. Wyman L., Goering R. V., Novick R. P. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics. 1974 Apr;76(4):681–702. doi: 10.1093/genetics/76.4.681. [DOI] [PMC free article] [PubMed] [Google Scholar]

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