Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Aug;167(2):587–593. doi: 10.1128/jb.167.2.587-593.1986

Linear plasmidlike DNA in the plant pathogenic fungus Fusarium oxysporum f. sp. conglutinans.

H C Kistler, S A Leong
PMCID: PMC212930  PMID: 3015880

Abstract

Double-stranded, 1.9-kilobase-pair (kbp) DNA molecules were found in 18 strains representing three pathogenic races of Fusarium oxysporum f. sp. conglutinans. The DNA element (pFOXC1) from a race 1 strain and the DNA element (pFOXC2) from a race 2 strain were shown by restriction endonuclease mapping to be linear. pFOXC2 was found in mitochondrial preparations and appears to have blocked 5' termini, as it was sensitive to 3'----5' exonuclease III but insensitive to 5'----3' lambda exonuclease. The major 1.8-kbp BglII restriction endonuclease fragment of pFOXC2 was cloned in plasmid pUC12. The recombinant plasmid (pCK1) was not homologous to the mitochondrial or nuclear genomes from F. oxysporum f. sp. conglutinans. This suggests that pFOXC2 is self-replicating. pCK1 was homologous to all 1.9-kbp DNA elements of race 2 but was not homologous to those of race 1 or race 5. All race 1 and 5 elements were also shown to share common DNA sequences.

Full text

PDF
587

Images in this article

588Image
on p.588
589Image
on p.589
589Image
on p.589
590Image
on p.590
590Image
on p.590
591Image
on p.591
591Image
on p.591

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Escarmís C., Gómez A., García E., Ronda C., López R., Salas M. Nucleotide sequence at the termini of the DNA of Streptococcus pneumoniae phage Cp-1. Virology. 1984 Feb;133(1):166–171. doi: 10.1016/0042-6822(84)90435-5. [DOI] [PubMed] [Google Scholar]
  2. Escarmís C., Salas M. Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1446–1450. doi: 10.1073/pnas.78.3.1446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garber R. C., Yoder O. C. Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components. Anal Biochem. 1983 Dec;135(2):416–422. doi: 10.1016/0003-2697(83)90704-2. [DOI] [PubMed] [Google Scholar]
  4. Hirochika H., Nakamura K., Sakaguchi K. A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs. EMBO J. 1984 Apr;3(4):761–766. doi: 10.1002/j.1460-2075.1984.tb01881.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Inman R. B., Schnös M. Partial denaturation of thymine- and 5-bromouracil-containing lambda DNA in alkali. J Mol Biol. 1970 Apr 14;49(1):93–98. doi: 10.1016/0022-2836(70)90378-5. [DOI] [PubMed] [Google Scholar]
  6. Lazarus C. M., Earl A. J., Turner G., Küntzel H. Amplification of a mitochondrial DNA sequence in the cytoplasmically inherited 'ragged' mutant of Aspergillus amstelodami. Eur J Biochem. 1980 May;106(2):633–641. doi: 10.1111/j.1432-1033.1980.tb04611.x. [DOI] [PubMed] [Google Scholar]
  7. Marriott A. C., Archer S. A., Buck K. W. Mitochondrial DNA in Fusarium oxysporum is a 46.5 kilobase pair circular molecule. J Gen Microbiol. 1984 Nov;130(11):3001–3008. doi: 10.1099/00221287-130-11-3001. [DOI] [PubMed] [Google Scholar]
  8. Meinkoth J., Wahl G. Hybridization of nucleic acids immobilized on solid supports. Anal Biochem. 1984 May 1;138(2):267–284. doi: 10.1016/0003-2697(84)90808-x. [DOI] [PubMed] [Google Scholar]
  9. Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
  10. Russell P. J., Wagner S., Rodland K. D., Feinbaum R. L., Russell J. P., Bret-Harte M. S., Free S. J., Metzenberg R. L. Organization of the ribosomal ribonucleic acid genes in various wild-type strains and wild-collected strains of Neurospora. Mol Gen Genet. 1984;196(2):275–282. doi: 10.1007/BF00328060. [DOI] [PubMed] [Google Scholar]
  11. Salas M. A new mechanism for the initiation of replication of phi 29 and adenovirus DNA: priming by the terminal protein. Curr Top Microbiol Immunol. 1984;109:89–106. doi: 10.1007/978-3-642-69460-8_4. [DOI] [PubMed] [Google Scholar]
  12. Singh L., Jones K. W. The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures. Nucleic Acids Res. 1984 Jul 25;12(14):5627–5638. doi: 10.1093/nar/12.14.5627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Specht C. A., DiRusso C. C., Novotny C. P., Ullrich R. C. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal Biochem. 1982 Jan 1;119(1):158–163. doi: 10.1016/0003-2697(82)90680-7. [DOI] [PubMed] [Google Scholar]
  15. Wright R. M., Cummings D. J. Integration of mitochondrial gene sequences within the nuclear genome during senescence in a fungus. Nature. 1983 Mar 3;302(5903):86–88. doi: 10.1038/302086a0. [DOI] [PubMed] [Google Scholar]
  16. Yoshikawa H., Ito J. Terminal proteins and short inverted terminal repeats of the small Bacillus bacteriophage genomes. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2596–2600. doi: 10.1073/pnas.78.4.2596. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES