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. 1998 Aug 4;95(16):9082–9086. doi: 10.1073/pnas.95.16.9082

Figure 2.

Figure 2

(a) Restriction map showing the predicted result of a double crossover between homologous regions of orf3 in pLRF25 and the S. clavuligerus chromosome. Solid box represents the tsr gene, open box represents the neomycin resistance gene, and the crosshatched box represents orf3. Abbreviations: B, BclI; C, ClaI; E, EcoRI; Ev, EcoRV; H, HindIII; K, KpnI; N, NcoI; P, PvuII; Sa, SacI; Sp, SphI. (b) Southern hybridization of S. clavuligerus chromosomal DNA from RFL35 and wild type with orf3-specific probe (probe 1) and tsr-specific probe (probe 2, after striping gel of probe 1). Lanes: 1, RFL35/BclI; 2, WT/BclI; 3, RFL35/SphI; 4, WT/SphI; 5, RFL35/KpnI–SacI; 6, WT/KpnI–SacI; Mr, λ/HindIII.