Alignment of homologous regions of AS and β-LS. Heavy shading is used to indicate conserved residues and light shading is used to indicate similarity. The first and second regions are closely linked to glutamine-dependent activity in AS, and the third region is associated with ATP binding. Human AS (human AS P08243), Rice AS (rice AS U55873), Myco tuber. AS (Mycobacterium tuberculosis), Eco K12 AS-B (Q10374 E. coli P22106), β-LS (β-LS from S. clavuligerus), CarA (CarA from Erwinia cartovora).