Skip to main content
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1976 Apr;76(2):287–298. doi: 10.1017/s0022172400055182

A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes.

G S Allan, R J Chappel, P Williamson, D J McNaught
PMCID: PMC2129629  PMID: 1063217

Abstract

Brucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis. IgM reacted more efficiently than IgG1 and IgG2 in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1 and did not react to IgG2. IgM was, however, partly inactivated when heated at 60 degrees C. in the presence of serum.

Full text

PDF
291

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton G. G., Maw J., Rogerson B. A., McPherson G. G. The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests. Aust Vet J. 1975 Feb;51(2):57–63. doi: 10.1111/j.1751-0813.1975.tb09404.x. [DOI] [PubMed] [Google Scholar]
  2. Beh K. J. Quantitative distribution of Brucella antibody amongst immunoglobulin classes in vaccinated and infected cattle. Res Vet Sci. 1974 Jul;17(1):1–4. [PubMed] [Google Scholar]
  3. Cho H. J., Ingram D. G. Mechanisms of prozone formation in agglutination reaction. Can J Microbiol. 1972 Apr;18(4):449–456. doi: 10.1139/m72-070. [DOI] [PubMed] [Google Scholar]
  4. Corbel M. J. Characterisation of antibodies active in the Rose Bengal plate test. Vet Rec. 1972 Apr 22;90(17):484–485. doi: 10.1136/vr.90.17.484. [DOI] [PubMed] [Google Scholar]
  5. Corbel M. J. Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis. J Hyg (Lond) 1972 Dec;70(4):779–795. doi: 10.1017/s0022172400022622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Corbel M. J. Studies on the mechanism of the Rose Bengal plate test for bovine brucellosis. Br Vet J. 1973 Mar-Apr;129(2):157–166. doi: 10.1016/s0007-1935(17)36540-5. [DOI] [PubMed] [Google Scholar]
  7. Curtain C. C. The influence of environment upon serum immunoglobulin levels in cattle. Br Vet J. 1971 Sep;127(9):442–450. doi: 10.1016/s0007-1935(17)37390-6. [DOI] [PubMed] [Google Scholar]
  8. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  9. Davies G. The Rose Bengal test. Vet Rec. 1971 Apr 24;88(17):447–449. doi: 10.1136/vr.88.17.447. [DOI] [PubMed] [Google Scholar]
  10. HESS W. R. Studies on a nonspecific Brucella-agglutinating substance in bovine serum. II. Isolation and purification of the Brucella-agglutinating substances. Am J Vet Res. 1953 Apr;14(51):195–197. [PubMed] [Google Scholar]
  11. Kulshreshtha R. C., Atal P. R., Wahi P. N. A study on serological tests for the diagnosis of human and bovine brucellosis. Indian J Med Res. 1973 Oct;61(10):1471–1477. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Marchalonis J. J. An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochem J. 1969 Jun;113(2):299–305. doi: 10.1042/bj1130299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller J. K., Nettleton P. F., Robertson A. M. Evaluation of a two-channel automated system for the sero-diagnosis of brucellosis. Vet Rec. 1973 May 12;92(19):492–496. doi: 10.1136/vr.92.19.492. [DOI] [PubMed] [Google Scholar]
  15. Morgan W. J. Brucellosis in animals: diagnosis and control. Proc R Soc Med. 1969 Oct;62(10):1050–1052. [PMC free article] [PubMed] [Google Scholar]
  16. ROSE J. E., ROEPKE M. H. An acidified antigen for detection of nonspecific reactions in the plate-agglutination test for bovine brucellosis. Am J Vet Res. 1957 Jul;18(68):550–555. [PubMed] [Google Scholar]
  17. Rice C. E., Boyes B. Serum immunoglobulins in bovine brucellosis. N Z Vet J. 1971 Jul;19(7):146–154. [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES