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. 1998 Aug 4;95(16):9105–9110. doi: 10.1073/pnas.95.16.9105

Figure 1.

Figure 1

Schematic representation of the constructs: P′-cytomegalovirus promoter, P-β-actin promoter, GFP-sequence coding for the green fluorescent protein, virD1- sequence coding for the VirD1 protein, virD2- sequence coding for the VirD2 protein, and A-simian virus 40 (SV40) polyadenylation signal. Regions coding for N- and C-terminal NLSs are represented by filled and opened bars, respectively. The corresponding amino acid sequences are indicated. pD2, wild-type virD2; pGFP-D2, wild-type virD2 fused to gfp; pGFP-PstD2, virD2 mutant, lacking the N-terminal NLS coding sequence and fused to gfp; pGFP-S132, virD2 mutant, lacking the C-terminal NLS coding sequence and fused to gfp; pGFP-S155, virD2 mutant, lacking both N- and C-terminal NLS coding sequences and fused to gfp; pGFP-D1, wild-type virD1 fused to gfp; pGFP-D2D1, three genes fused in frame: gfp, wild-type virD2, and wild-type virD1.