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. 1998 Aug 4;95(16):9105–9110. doi: 10.1073/pnas.95.16.9105

Figure 3.

Figure 3

Subcellular localization of the VirD2 NLS mutant proteins fused to GFP, in HEK 293 cells. (A) Wild-type VirD2 protein fused to GFP (cells were transfected transiently with pGFP-D2). (B) N-terminal NLS mutant VirD2 protein (cells were transfected transiently with pGFP-PstD2. (C) C-terminal NLS mutant VirD2 protein (cells were transfected transiently with pGFP-S132). (D) Mutant VirD2 protein lacking both N- and C-terminal NLS (cells were transfected transiently with pGFP-S155). Cells were fixed and stained with propidium iodide.