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. 1998 Aug 4;95(16):9129–9133. doi: 10.1073/pnas.95.16.9129

Figure 2.

Figure 2

Thermal stability of an E. coli crude extract expressing Mj HSP16.5. Extract of soluble E. coli proteins from induced BL21(DE3)/pSJS1240 and BL21(DE3)/pSJS1240 cells expressing Mj HSP16.5 were prepared as described in Materials and Methods. Aliquots of cell extract (4 mg/ml) were heated for 20 min at various temperatures, as indicated. After being allowed to cool to room temperature, samples were centrifuged at 10,000 × g for 5 min. The supernatants (10 μl) were analyzed by SDS/PAGE. Lanes: 1–7, BL21(DE3)/pSJS1240 cell extract heated at 20°, 60°, 70°, 80°, 90°, 100°, and 110°C, respectively; 8, molecular mass markers in kilodaltons; 9–15, transformed BL21(DE3)/pSJS1240 cell extract expressing Mj HSP16.5 heated at 20°, 60°, 70°, 80°, 90°, 100°, and 110°C, respectively.