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. 1998 Aug 4;95(16):9140–9145. doi: 10.1073/pnas.95.16.9140

Figure 5.

Figure 5

Functional expression of murine CMP-Neu5Ac synthetase in E. coli mutant EV5. E. coli EV5 cells were transformed with pQEAM16, encoding the murine CMP-Neu5Ac synthetase, or empty vector pQE30 or pUE17, containing the CMP-Neu5Ac synthetase from N. meningitidis. Expression of the transformed DNA was induced with 0.2 mM isopropyl-β-d-thiogalactoside, and the presence of PSA capsule was determined by Western blot analysis by using anti-PSA mAb 735. PSA was removed specifically if samples were treated with endoNE (+).