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Reconstitution of CMP–Neu5Ac synthetase activity in pAM15 transfected LEC29.LEC32 cells
| Cell line | Plasmid | Activity, nmol/min/mg protein |
|---|---|---|
| wt CHO | — | 1.98 |
| LEC29.Lec32 | — | 0.006 |
| LEC29.Lec32 (pool) | pABE | 0.023 |
| LEC29.Lec32 (pool) | pAM15 | 0.51 |
CMP-Neu5Ac synthetase activity was determined in wt CHO and LEC29.Lec32 cells before and after stable transfection with the murine CMP-Neu5Ac synthetase cDNA pAM15 or the empty vector pABE.
