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Effect of GSH and modification of lysines on ATP binding to MT. ATP (100 μM) was incubated with rabbit Cd,Zn-MT-2 (142 μM) in 50 mM Hepes⋅NaOH (pH 7.4) in the absence of GSH or in the presence of either GSH (120 μM) or GSSG (500 μM) (4). Binding was determined by the Hummel–Dreyer method.
