Skip to main content
PMC home page
The Journal of Hygiene logoLink to The Journal of Hygiene
. 1971 Mar;69(1):73–82. doi: 10.1017/s0022172400021264

Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits

Janet M Bradbury, F T W Jordan
PMCID: PMC2130842  PMID: 5291753

Abstract

Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.

However, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.

Full text

PDF
76

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARGAMAN M., RAZIN S. CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA. J Gen Microbiol. 1965 Jan;38:153–160. doi: 10.1099/00221287-38-1-153. [DOI] [PubMed] [Google Scholar]
  2. BUTLER M., KNIGHT B. C. The survival of washed suspensions of Mycoplasma. J Gen Microbiol. 1960 Apr;22:470–477. doi: 10.1099/00221287-22-2-470. [DOI] [PubMed] [Google Scholar]
  3. EDWARD D. G., FITZGERALD W. A. Cholesterol in the growth of organisms of the pleuropneumonia group. J Gen Microbiol. 1951 Aug;5(3):576–586. doi: 10.1099/00221287-5-3-576. [DOI] [PubMed] [Google Scholar]
  4. EDWARD D. G., FITZGERALD W. A. The isolation of organisms of the pleuropneumonia group from dogs. J Gen Microbiol. 1951 Aug;5(3):566–575. doi: 10.1099/00221287-5-3-566. [DOI] [PubMed] [Google Scholar]
  5. EDWARD D. G., FREUNDT E. A. The classification and nomenclature of organisms of the pleuropneumonia group. J Gen Microbiol. 1956 Feb;14(1):197–207. doi: 10.1099/00221287-14-1-197. [DOI] [PubMed] [Google Scholar]
  6. ENNY G. E., GRAYSTON J. T. EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS. J Immunol. 1965 Jul;95:19–25. [PubMed] [Google Scholar]
  7. Fernald G. W., Clyde W. A., Jr, Denny F. W. Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera. Proc Soc Exp Biol Med. 1967 Oct;126(1):161–166. doi: 10.3181/00379727-126-32391. [DOI] [PubMed] [Google Scholar]
  8. Friedrich H. Zur Anwendung des Stoffwechselhemmtests (MI-test) bei Mycoplasma gallisepticum. Dtsch Tierarztl Wochenschr. 1970 Jan 1;77(1):12–14. [PubMed] [Google Scholar]
  9. Jordan F. T., Kulasegaram P. Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum. J Comp Pathol. 1968 Oct;78(4):407–414. doi: 10.1016/0021-9975(68)90038-8. [DOI] [PubMed] [Google Scholar]
  10. KELLNER A., CORRELL J. W., LADD A. T. Sustained hyperlipemia induced in rabbits by means of intravenously injected surface-active agents. J Exp Med. 1951 Apr 1;93(4):373–384. doi: 10.1084/jem.93.4.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MORAN P. A. Another test for heterogeneity of host resistance in dilution assays. J Hyg (Lond) 1958 Sep;56(3):319–322. doi: 10.1017/s0022172400037815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MORAN P. A. The dilution assay of viruses. J Hyg (Lond) 1954 Jun;52(2):189–193. doi: 10.1017/s002217240002739x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RODWELL A. W., ABBOT A. The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides. J Gen Microbiol. 1961 Jun;25:201–214. doi: 10.1099/00221287-25-2-201. [DOI] [PubMed] [Google Scholar]
  14. RODWELL A. W. THE STEROID GROWTH-REQUIREMENT OF MYCOPLASMA MYCOIDES. J Gen Microbiol. 1963 Jul;32:91–101. doi: 10.1099/00221287-32-1-91. [DOI] [PubMed] [Google Scholar]
  15. SEARCY R. L., BERGQUIST L. M. A new color reaction for the quantitation of serum cholesterol. Clin Chim Acta. 1960 Mar;5:192–199. doi: 10.1016/0009-8981(60)90035-8. [DOI] [PubMed] [Google Scholar]
  16. SMITH P. F., LECCE J. G., LYNN R. J. A lipoprotein as a growth factor for cortain pleuropneumonialike organisms. J Bacteriol. 1954 Nov;68(5):627–633. doi: 10.1128/jb.68.5.627-633.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SMITH P. F. Nutritional requirements of PPLO and their relation to metabolic function. Ann N Y Acad Sci. 1960 Jan 15;79:508–520. doi: 10.1111/j.1749-6632.1960.tb42717.x. [DOI] [PubMed] [Google Scholar]
  18. Smith S. C., Dunlop W. R., Strout R. G. Effect of culture medium on antigenic structure of mycoplasma. Avian Dis. 1966 May;10(2):173–176. [PubMed] [Google Scholar]
  19. TAYLOR-ROBINSON D., SOMERSON N. L., TURNER H. C., CHANOCK R. M. SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION. J Bacteriol. 1963 Jun;85:1261–1273. doi: 10.1128/jb.85.6.1261-1273.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor-Robinson D., Purcell R. H., Wong D. C., Chanock R. M. A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production. J Hyg (Lond) 1966 Mar;64(1):91–104. doi: 10.1017/s0022172400040377. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Hygiene are provided here courtesy of Cambridge University Press

RESOURCES