Figure 4.

Effects of glucose (A) and galactose (B) on cell growth of nontransfected and NDI1-transfected CCL16-B2 and parent CCL16. (A) Parent CCL16 cells (10⁵) (□), nontransfected CCL16-B2 cells (▵), and NDI1-transfected CCL16-B2 cells (○) were inoculated in a 5-mM glucose culture medium. (B) Nontransfected (triangles) and NDI1-transfected cells (10⁵) (circles) were inoculated in a 0.6-mM glucose culture medium in the presence (closed symbols) and absence (open symbols) of 5 mM galactose. In the case of NDI1-transfected CCL16-B2 cell culture, 0.5 mg/ml of antibiotic G418 also was present. Cells were cultured at 37°C in a 5% CO₂ atmosphere. Cell viability was assessed by trypan blue exclusion, and cell numbers were determined every 24 hr by using a hemocytometer.