Figure 5.

Transport of 10-kDa Calcium Green-1 dextran into intact isolated nuclei. (A) Micrographs showing Ca²⁺-dependent loading of nuclear preparation with 10-kDa Calcium Green-1 dextran under control and PKA-phosphorylated conditions. For each condition, fluorescence (λ_ex ≈ 488 nm, λ_em ≈ 531 nm, Upper) and corresponding phase-contrast (Lower) micrographs are shown. (B) Quantification of confocal fluorescence micrographs. Background fluorescence values were subtracted. Data are presented as mean values ± SEM. Statistical significance was assessed by using the Mann–Whitney U test. (∗, P < 0.05, control vs. PKA).