Table 1.
Marker enzyme activity
| Marker enzymes | Liver homogenate
|
Nuclei
|
||
|---|---|---|---|---|
| Sp. act. | Total act. | Sp. act. | Total act. | |
| NAD pyrophosphorylase | 4.04 | 27.3 | 24.3 | 7.06 |
| Mannose-6-phosphatase | 93.3 | 628.0 | 332.0 | 122.0 |
| NADPH cytochrome c reductase | 9.5 | 57.0 | 3.2 | 0.8 |
| 5′-Nucleotidase | 4.1 | 20.5 | 0.21 | 0.04 |
| Cytochrome c oxidase | 12.0 | 60.0 | 0.03 | 0.066 |
NADPH cytochrome c reductase activity was determined by monitoring the reduction of cytochrome c at 550 nm. NAD pyrophosphorylase activity was determined by monitoring the formation of NADH at 340 nm. Mannose-6-phosphatase activity was determined by measuring the absorbance at 730 nm. 5′-Nucleotidase activity, cytochrome c oxidase activity, and NAD pyrophosphorylase activity were determined as described by Masmoudi et al. (23), Malviya et al. (2), and Humbert et al. (6), respectively. Specific activity (sp. act.) is expressed as nmol/min per mg of protein, and the total activity is expressed as μmol/min.