Figure 4.

Intracellular location of the cell-permeable Scβ₃-1A peptide as demonstrated by confocal laser scanning microscopy. Intracellular peptide was detected as yellow fluorescence and analyzed by a four-step Z-position sectional scanning of the cell (1-μm sections). (A) Composite image of an untreated cell. (B) Composite image of a cell treated with β₃-1 peptide (lacking a MPS). (C) Composite image of a cell treated with thiazolidine-ligated Scβ₃-1A peptide. (D–G) Sequential Z-position sections of the Scβ₃-1A peptide-treated cell showing distribution of the peptide throughout the cytoplasm and cell membrane.