Figure 5.

Nuclear import inhibition by the cell-permeable p50 NLS peptides. Jurkat T cells were treated for 30 min with the peptides (75 μM) indicated above each lane (see Table 1). Cells were then stimulated with PMA (5 nM) and ionomycin (2 μM) for 60 min. Nuclear extracts were prepared and assayed for the presence of each transcription factor by electrophoretic mobility shift assay as described in Materials and Methods. Only the section of each autoradiogram that includes the specific transcription factor–³²P-labeled DNA complex is shown as indicated for NF-κB, AP-1, NFAT and the constitutively nuclear transcription factor NF-Y.