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. 1998 Aug 4;95(16):9190–9195. doi: 10.1073/pnas.95.16.9190

Figure 4.

Figure 4

E2F-6 contains a transferable repression domain. (A) Five micrograms of a luciferase reporter gene with multiple Gal4 binding sites was cotransfected with the indicated amounts of Gal4-RB or Gal4-E2F-6. (B) Five micrograms of the Gal4 luciferase reporter was cotransfected with 0.1 μg of the indicated Gal4-E2F-6 fusion protein expression vectors. (C) Schematic representation of the Gal4 fusion vectors used in A and B. (D) Five micrograms of E2F-luciferase reporter gene was cotransfected with 0.5 μg of empty vector (ctrl) or 0.5 μg of the indicated E2F-6 expression vectors. (E) Schematic representation of the E2F-6 mutants used. (F) Western blot analysis using an anti-HA antibody, showing that all E2F-6 proteins are expressed at comparable levels. In all reporter assays, 0.2 μg of CMV-β-gal was cotransfected, and luciferase activity was normalized to β-galactosidase activity.