Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
KMnO4 footprinting; pol III assembled by TFIIIB[B′′Δ355–372] does not open the promoter. TFIIIB containing full length B′′ or B′′Δ355–372 was bound to a 132-bp U6LboxB-derived DNA probe with 5 (1×) or 10 (2×) fmol pol III or without pol III as indicated above each lane. For the samples in lanes 5 and 9, GTP, UTP, and CTP were added to allow formation of 7-mer nascent transcripts. Thymine residues oxidized by MnO4− are marked at the side (sequence in Fig. 1a).
